Improved Lentiviral Vector (LVV) Purity With Multi-Modal Chromatography

Our previous post on Considerations for Downstream Processing provides an overview of each unit operation. A critical aspect of scaling-up production of LVV is the optimization of each unit operation in downstream processing (DSP) to improve the recovery of a purified and concentrated virus.

While some degree of purification is achieved in every unit operation, polishing involves the removal of major impurities such as residual host cell DNA and protein. This post examines the utility of multi-modal chromatography as a novel strategy during polishing to improve LVV purity.