How To Scale-Up Lentiviral Vector Production Part 2: Considerations For Downstream Processing

In Part 1 of our series on lentiviral vector (LVV) manufacturing we covered scale-up of upstream processing steps. In this post we will look at the key steps in downstream processing (DSP). The industry “gold-standard” for recovery of purified and concentrated LVV is 10-20 percent. Improving on this low recovery is an opportunity to reduce the cost of manufacturing and get viral vectors into the hands of researchers who need them.

Below we break down DSP into unit operations and understand how process innovations are contributing to scalability at each step.