Article | May 30, 2023

How To Prepare Your Specimen For Immunofluorescence Microscopy

By Florian Hoff, Leica Microsystems


Immunofluorescence (IF) has become indispensable for many research groups who have at least access to a simple fluorescence microscope. It is a powerful method for visualizing intracellular processes, conditions and structures. The centerpiece of an Immunofluorescence (IF) experiment is a combination of two different components:

  • First, specific antibodies, which are used to form an immune complex to mark the desired molecules – in most cases proteins – in the cell.
  • Second, fluorochromes, which are coupled to the immune complexes and therefore visualize the target structures during microscopy.

Explore Immunofluorescence preparations and protocols along with it's advantages and limitations.

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