Highly Sensitive ELISAs In 90 Minutes: SimpleStep ELISA® Kits And The SPECTROstar® ^Nano

By Mallori Milligan, Abcam, Scott Detmer, Abcam, and Carl Peters, BMG LABTECH

This content is brought to you by Abcam, a Danaher Operating Company.

Since its development in the 1970s, the enzyme-linked immunosorbent assay (ELISA) has established itself as one of the most trusted and widely used biochemical assays. Its ability to detect and quantify analytes has made it an essential diagnostic tool in medicine and research applications across a majority of biological disciplines.

However, traditional ELISA methods are often limited by their relatively low throughput and the need for multiple wash steps, which typically span 3 to 5 hours. SimpleStep ELISA® technology from Abcam overcomes these challenges by simplifying the procedure into a semi-homogeneous format, reducing the protocol to a 90-minute, single-wash.

Examine the detection of this innovative ELISA using the SPECTROstar®^Nano. Paired with MARS data analysis, this method provides results quickly and easily.