High-Resolution Characterization Of Both sgRNA And Cas9 mRNA In A Single Analysis For CRISPR/Cas9 Applications

By Mario A. Pulido, Mervin Gutierrez, Fang Wang, Jane Luo and Marcia Santos

This content is brought to you by SCIEX, a Danaher Operating Company.

Overcome the challenges of accurately characterizing and quantifying differently sized RNA molecules, impurities, and degraded RNA species in vaccines. The RNA 9000 Purity & Integrity Kit improves your characterization of both RNA integrity in the final product and RNA fragmentation with an analytical kit-based solution that can separate single-stranded RNA products in the range of 50 to 9,000 bases.

Key features of the kits include:

• Ability to acquire high-resolution data for RNA fragments ranging from 50 to 9,000 bases using the RNA 9000 Purity & Integrity Kit
• Excellent %CV, < 2%, for the corrected peak area% of the main product of the Cas9 mRNA component of the CRISPR/Cas9 gene editing system
• Ready-to-use, validated kit and methods enable efficient RNA fragment characterization using the high-throughput BioPhase 8800 system