Frequently Asked Questions: Corning® CellCube® System

The Corning CellCube system is a method for mass culture of attachment-dependent cells. It consists of CellCube modules, which are polystyrene plates joined together to create laminar flow spaces for the flow of media. The system allows for consistent distribution of nutrients and oxygen throughout the module. The CellCube system can be used with different types of bioreactors and controllers as long as they have the appropriate ports and connectors.

To choose the appropriate size and surface treatment for the CellCube module, factors such as cell type, application, and process need to be considered. The modules are available in 10-, 25-, and 100-layer options with both Tissue Culture (TC)-treated and Corning CellBIND surfaces.

The working volume of each CellCube module is fixed based on the surface area, but the volume needed in the bioreactor and circulation loop can be tailored for each application. The system can be used for seed train by harvesting cells from one module to seed new modules. The modules can be coated with a specific coating process and cells can be seeded by rotating the modules into a vertical position.

The recommended medium conditioning and gassing parameters vary depending on the cell type and medium formulation. The recommended circulation flow rate during cell expansion is dependent on the cell type and module size. Cells can be harvested from the CellCube modules using cell dissociation reagents.

The CellCube system can be used to culture a wide range of adherent cells for various applications, including cell and gene therapy. It allows for transient transfections and is scalable by increasing the number of layers or modules.