Flow Cytometry Analysis Of Chemokine Receptors Expressed On Human NK Cells

By Mieszko Lachota, Medical University of Warsaw

Natural killer (NK) cells are effector cells of the innate immune system, defined as CD56+ and CD3– lymphocytes, capable of exerting natural cytotoxicity and antibody-dependent cytotoxicity (ADCC). These cells play an important role in cancer surveillance due to their unique ability of spontaneous cytotoxicity against cancer cells, which also makes them an attractive tool for immunotherapy. This application note describes the analysis of the human chemokine receptor repertoire by flow cytometry. We present an antibody panel allowing simultaneous assessment of two chemokine receptors on both CD56^bright and CD56^dim NK cells that were either rested or stimulated with IL-15.

Learn how the knowledge generated by a deep and thorough analysis of chemokine receptors on NK cells can be helpful to scientists.