Fast And Efficient T Cell Isolation Without Density Gradient Centrifugation For CAR T Cell Engineering

In recent years, CAR T cell-mediated immunotherapy has shown high clinical relevance in the fight against cancer. However, increased research efforts to address challenges, such as poor efficacy and unwanted side effects, are required to further advance the field. Miltenyi Biotec has established optimized CAR T cell engineering workflows, combining cell isolation, culture, and analysis tailored for CAR T cell generation in different contexts.

Follow along through a side-by-side comparison of StraightFrom MicroBeads and standard MicroBeads for the generation of CAR T cells. After T cell isolation, the immune cell composition, T cell activation status, transduction efficiency, and CAR T cell functionality were compared. By using the isolation of T cells directly from blood products, the experimental procedure is accelerated and allows for automation on platforms while maintaining the quality, functionality, and downstream compatibility of the isolated T cells.