Expression Of Recombinant Proteins

A series of scientific discoveries – unraveling the genetic code, isolation of restriction enzymes and DNA ligases, and expression of foreign genetic material in heterologous systems – positively impacted Science and Medicine. These advances culminated in the ability to express and purify large amounts of recombinant proteins.

Expression of recombinant proteins revolutionized basic research and clinical practice. Initially employed to resolve three-dimensional structures of proteins, this technique evolved to allow the development of innovative diagnostic and therapeutic applications. The combination of flexibility offered by the technique with high-quality reagents leads to the expression of functional proteins for diverse applications. The creativity of research teams is now the limiting factor.

In this article we highlight state-of-the-art reagents that are solving challenges previously faced in the expression process.