Expansion Of Vero Cells On Corning® Enhanced Attachment Microcarriers And Demonstration Of Scalability In A Benchtop Bioreactor

By Jeffery J. Scibek and Kathleen A. Krebs, Corning Incorporated, Life Sciences

To address the need for increased cell yields, Corning has commercialized a panel of sterile, ready-to-use microcarriers for the culture of anchorage-dependent cell lines. Corning microcarriers are available with a variety of surface treatments to support the growth of different cell types. Microcarriers provide an ideal platform for improving the cell yield of anchorage-dependent cell lines by significantly increasing the culture surface area-to-volume ratio. Corning microcarriers provide many of the same advantages as traditional beads with the added benefit of being sterile and ready-to-use, which eliminates the need for time-intensive swelling, washing, and sterilization that is necessary when using other microcarriers.

Here we report on the expansion and scale-up of Vero cells on Corning enhanced attachment microcarriers for vaccine applications. Vero cells are an ideal candidate for microcarrier culture because they are anchorage-dependent and are required in large numbers for cell line-based vaccine production.