EV Content vs. Function: Using Analytical Tools To Reveal The Connection

Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) show therapeutic promise, but their complexity demands rigorous analytical characterization throughout manufacturing. This webinar demonstrates how quantitative western blot, nanoflow cytometry, and functional enzymatic assays work together to measure CD73, a critical quality attribute on MSC-EVs.

Listen in as RoosterBio experts present data showing how different downstream processing steps impact both CD73 content and enzymatic function. The presentation reveals strong correlations between CD73 expression levels and biological activity across harvest, filtration, and concentration stages. You'll learn how larger particles with high CD73 density are affected by initial filtration, while remaining CD73-positive vesicles maintain functionality through subsequent processing. You'll also learn about establishing activity-coupled biomarkers that link EV composition to potency, a framework applicable beyond CD73 to other critical quality attributes.

Check out the session below to understand how analytical characterization informs manufacturing choices and supports the transition from research-scale development to clinical production of EV-based therapeutics.