Traditional cell line development workflows are commonly time consuming, labor intensive lacking quality data control. The Solentim Ecosystem encompasses the VIPS, Cell Metric and ICON instrumentations alongside the STUDIUS data management platform. The development of these cutting-edge technologies enables automated and streamlined workflows, facilitating the isolation and characterization of high value cells. We will outline how the range of technologies support single-cell seeding, image based clonality assurance and allow for informed analysis of critical, cell quality attributes. Further, we will focus on the importance of repeatable and trackable data to ensure compliance with regulatory bodies.