Efficient Serum-Free Cryopreservation Of Primary Cells And Tumors With MACS® Freezing Solution

Cryopreservation of cells and solid tissues is a common lab practice for the long-term preservation and delayed use of samples. This procedure is fairly straightforward; cell or tissue samples are slowly frozen in a cryopreservation medium, stored in liquid nitrogen, and thawed quickly when they are needed. The cryopreservation medium creates a supportive environment for the cells that prevents the formation of ice crystals, which would otherwise damage the cell membrane, and enables the cells and tissues to recover after thawing. Therefore, the composition of the cryopreservation medium can have a big impact in the recovery of viable cells after thawing. This is especially true for primary cells, which are rather sensitive and demand a more supportive medium to survive the freeze-thaw process.

A chemically defined, serum-free, and animal component–free cryopreservation medium was developed, taking into consideration the special needs of primary cells and tissues during freeze-thawing. Explore the performance of this freezing solution in the cryopreservation of peripheral blood mononuclear cells (PBMCs), mouse tumors, and tumor cell suspensions.