Efficient-Pro Medium And Feeds Improve Productivity In CHO-K1, CHO-S, And DG44 Cells

Year after year, biopharmaceutical manufacturers have successfully driven their CHO cells to deliver increased productivity. Despite these advances, the industry continues to strive toward maximizing productivity by streamlining manufacturing processes to reduce costs. To maximize productivity and reduce development time and costs, CHO workflows in the early stages of development require a higher-performing chemically defined (CD) platform with basal media and feeds optimized for specific CHO cell lines.

To help provide solutions for the bioproduction industry, the Gibco™ Efficient-Pro™ Medium and Gibco™ Efficient-Pro™ Feeds 1 and 2 were developed using a traditional and multiomics modeling approach to improve recombinant protein production in CHO cells. In this application note, the performance of Efficient-Pro Medium and Feeds was evaluated using CHO-K1, CHO-S, and DG44 cells and compared to another supplier’s commercially available basal medium and 2-part feed.