Efficiency Of jetOPTIMUS® In Hard-To-Transfect Stem Cells: Mesenchymal And Pluripotent Cells To the Test

By Thibaut Benchimol, Alengo Nyamay’antu, and Claire Gueguen

The biggest challenge when transfecting stem cells is cell death which has been reported after both non-viral and viral transfection delivery methods⁽²⁾. Furthermore, as a result of both susceptibility to cell death and fast self-renewal, transfection efficiency can be lower with stem cells than with other cell types.  

In order to unleash the benefits of genetic manipulation with a chemical-based transfection, the ideal reagent would need to achieve high transfection efficiency (in the case of stem cells above 50%) while minimising any cell viability issues common with stem cells. Here we report the transfection efficiency performance of our chemical-based transfection reagent in three hard-to-transfect stem cells: mesenchymal stem cells (hMSCs), mouse embryonic stem cells (mESCs) and human induced pluripotent stem cells (hiPSCs). 

Discover how this transfection reagent demonstrates robust, non-toxic and efficient gene expression delivery in hard-to-transfect primary hMSCs, mESCs and induced pluripotent stem cells.