Dissociation Of Melanoma Tumors For The Generation Of Tumor-Infiltrating Lymphocyte Cultures For Adoptive Cell Therapy

By Linda L. Parker, Olaf Hardt, Alexander Scheffold, Andreas Bosio, and Mark E. Dudley

Tumor-infiltrating lymphocytes (TILs) are immune cells that have infiltrated a tumor and can be used in adoptive cell therapy (ACT) to help fight the tumor. To obtain TILs, melanoma tumors are dissociated using a variety of methods. One common method is to use enzymes to break down the tumor tissue. The enzymes are then removed, and the resulting cell suspension is filtered to remove debris. The filtered cell suspension is then cultured in vitro to expand the number of TILs. This experiment used multiple variables to examine the gentleMACS Dissociator for the generation of TIL cultures for research and clinical use.

Find out what an optimal standard operating procedure (SOP) is for the preparation of tumor-infiltrating lymphocytes and the minimum number of variables to optimize.