Application Note

Diploid Serum-Reduced Medium System Supports Viral Production And Outperforms In Specific Productivity


The Gibco™ Diploid Serum-Reduced Medium (SRM) system was developed for diploid cells in order to minimize the use of fetal bovine serum (FBS) in vaccine production and overcome cultivation challenges by supporting cell growth without medium adaptation. The system consists of Gibco™ Diploid Growth SRM for cell culture and Gibco™ Diploid Production Serum-Free Medium (SFM) for virus production. In a recent application note, Diploid Growth SRM was shown to allow recovery and expansion of diploid cells without adaptation, while reducing serum use up to 90%.

Here we present a study that was conducted to evaluate the growth and production capabilities of the Diploid SRM system with MRC-5 cells. MRC-5 cell expansion was compared in Diploid SRM and three alternative media from other suppliers over a cultivation period of eight passages. In addition, varicella zoster virus (VZV) and vesicular stomatitis virus (VSV) titer and specific productivity were evaluated with the Diploid SRM system and with classical media.

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Thermo Fisher Scientific Bioproduction