Developing A Microcarrier Stirred Tank Process For Large-Scale hMSC-EV Production

By Stephen Lenzini, James Brennan, Elie Zakhem & Jon Rowley

One of the challenges in EV production is scaling up the manufacturing processes using the optimal cell source and collection media. EV productivity is determined by the final cell yield and the number of EVs generated per cell. Therefore, increasing final cell yield as a first step will increase EV productivity.

In this study, we successfully developed and optimized a process to maximize hMSC yield using RoosterNourish™ and microcarriers in a stirred tank bioreactor system.