Cryopreservation of cells and cell lines is a key technique in 3D cell culture. It preserves cells, tissues, organoids, and other biological constructs by cooling the cells to extremely cold temperatures.
Cell cultures such as organoids comes with significant challenges. Long-term storage of organoids would allow for continued organoid technology development and clinical translation, but ice crystal formation makes freezing complex tissues difficult. Maintaining cell viability through ice formation and thawing is critical.
Here we review a few best practices to avoid damage during cryopreservation.