Cracking The Reproducibility Code: Sera Is A Key Component

Cracking the code to reproducible cell culture experiments has long been a challenge for researchers. A key, yet often overlooked, component is fetal bovine serum (FBS). While crucial for cell health and growth, FBS introduces significant variability due to its complex and undefined composition. Derived from cow blood, its characteristics are influenced by a myriad of factors, from the animal's diet and location to the time of year and even the gestational age of fetal calves. This inherent lot-to-lot variability can significantly impact experimental outcomes, hindering reproducibility and casting doubt on research findings.

While serum-free media remains a long-term goal, FBS continues to be a vital tool for many cell types. However, the challenges of FBS variability can be mitigated. Careful selection of serum treatments, rigorous testing of different lots, and strategic stockpiling are crucial steps towards ensuring consistent and reliable results. Choosing the right supplier, one with a vertically integrated and traceable supply chain, is equally important.

Learn how to navigate the complexities of FBS selection and usage to improve your experimental reproducibility and boost your confidence in your research. Read the full article to learn more.