By Hilary Sherman and John Shyu, Corning Incorporated, Life Sciences
Research and therapies involving the human immune system rely heavily on peripheral blood mononuclear cells (PBMCs) isolated from whole blood sources. Typical methods of PBMC isolation involve density gradient centrifugation or expensive, fully automated systems. Although density gradient centrifugation is often employed, the process is labor-intensive, has lower sample volume capacity, and is not easily adaptable to closed or automated systems. The Corning X-LAB system is designed to isolate and harvest PBMCs from blood and blood products in a closed, sterile device that is automation-friendly. Here we demonstrate high recovery and purity of PBMCs from 240 mL of whole blood from multiple donors using the Corning X-LAB system. Our results show that the X-LAB system can deplete more than 99% of red blood cells and 80% of platelets.