Cell Contamination, Containment, And Correction: Good Cell Culture Practices In 3D

The major causes of contamination in the cell culture system are typically either chemical or biological. In 3D cell culture, contamination can occur in various components, such as the bio-ink or the extracellular matrix. Most chemical contaminants are found in the cell culture media and originate from either reagents, the water used to make the reagents, or additives to supplement reagents. Biological contaminants often enter cell cultures by use of nonsterile supplies and media. Sterilization procedures using autoclaving or dry heat sterilization must be followed properly.

What are the proper post-contaminant procedures?