CE-SDS Analysis Of Etanercept Using The BioPhase 8800 System

By Zhichang Yang, Jane Luo, Marcia Santos, Sahana Mollah, SCIEX, USA

Capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) is one of the most powerful separation techniques in the analytical chemistry toolbox because it offers a high speed of analysis, high resolution, and quantification. In this study, the BioPhase 8800 system was used to analyze etanercept by CE-SDS. The multi-capillary capability of the BioPhase 8800 system allowed for the simultaneous analysis of etanercept under different deglycosylation reaction conditions, deglycosylation time-course, and assessing the consistency of deglycosylation reactions in other lots, accelerating methods development for protein analysis.

Key features of the BioPhase 8800 System include:

• Multi-capillary separation on the BioPhase 8800 system enables simultaneous analysis of multiple etanercept samples prepared under different conditions (reduction and deglycosylation)
• Pre-assembled cartridge and ready to use CE-SDS kit and consumables simplifies operation
• A time-course approach was used to help determine the optimal deglycosylation incubation periods for etanercept. The study indicates complete deglycosylation of etanercept was achieved within 1.5 hours.
• The deglycosylation reaction conditions were applied to other lots to quickly evaluate the reaction consistency, made possible with the parallel processing capabilities of the BioPhase 8800 system, significantly shortening the overall analysis time