CAR Detection Reagents - Applications For Flow Cytometry, Magnetic Cell Enrichment, And Microscopic Imaging

By Stephan Wilmes, Desiré Missing, Anja Brauchle, Nadine Borgelt, Johanna Müllers, Monika Winkels, Larissa Möckel, Michaela Niemöller, Katrin Lange, Laura Meinersen, Dagmar Häußler-Kuballa, Franziska Antonia Bertram, Olga Goldbach, Dominik Poschen, Astrid Imann, Anne Bodenhausen, Silvia Rüberg, Anijutta Appelshoff er, Fabio El Yassouri, Christian Dose, Marsilius Mues, and Anne Richter

Advances in CAR T cell therapy have heightened the need for precise, reproducible methods to detect and analyze engineered immune cells across research, development, and clinical workflows. A versatile set of detection reagents enables consistent identification of CAR expression using both antigen-based constructs and anti-idiotype antibodies, each designed to minimize background signal while maintaining high specificity. Flexible conjugation options support multiparameter flow cytometry panels, while integrated workflows extend beyond analysis to include magnetic enrichment of rare CAR-positive populations and automated gating for reproducible results. These capabilities also translate into imaging applications, allowing clear visualization of CAR T cell infiltration within tissue and enabling deeper phenotypic insight.

Explore how streamlined detection, isolation, and imaging approaches can strengthen CAR T cell characterization from early discovery through translational research.