Assay For Rapid Lot-Release Testing In Cell Therapies

By Kathleen Dunphy, Paromita Deb, Angel Zhao, Qi Dang, Michael Mateling, Mihir Rajhansa, Judy Zhu, Michael Afara, Sharon Rouw, and Despina Lymperopoulou, Thermo Fisher Scientific

Learn how PCR-based Mycoplasma detection assays can provide a rapid alternative to culturing with comparable sensitivity, specificity, and species range that can facilitate quick decision-making. The assay used in this experiment specifically targets the 16S rDNA sequence conserved across a vast range of Mycoplasma strains including three priority groups in the presence of multiple matrices.

The Applied Biosystems™ MycoSEQ™ Plus Mycoplasma Detection Assay, a TaqMan-based PCR multiplex Mycoplasma detection assay that can detect over 200 species of Mycoplasma targets, avoid false positives, and monitor PCR inhibition in a single reaction. Additionally, the assay includes a multi-purpose Discriminatory Positive Control designed to evaluate DNA extraction efficiency during sample preparation, and discriminate between a true positive-sample and an accidental cross-contamination with the Positive Control. The assay is designed for use with the Applied Biosystems™ PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit, and with the Applied Biosystems™ PrepSEQ™ Express Nucleic Acid Extraction Kit, in both an automated and a manual workflow and is supported by validated templates in AccuSEQ™ software.