Antigen-Specific Restimulation Of CD8+ T Cells By Cross-Presenting Dendritic Cells

The cross-priming capacity of dendritic cells (DCs) is essential for the initiation of effective cytotoxic T cell (CTL) responses against tumors and, thus, an intense study object in immuno-oncology. In this context, co-cultures of cross-presenting DCs (XP-DCs) and CD8+ T cells in the presence of model antigens with high prevalence in healthy donors are performed to better understand the underlying immune mechanisms and potential therapeutic applications. However, high-quality automated cell isolation, as well as consistent cell culture and stimulation conditions with easy translation into clinics, are required for successful progression of such studies.

The fast and easy automated isolation of fully functional CD8+ T cells and XP-DCs with the autoMACS® Pro Separator, can be easily translated into clinics using MACS® GMP cell isolation and cell culture reagents, in addition to fully closed cell processing platforms. See how CD141 XP-DCs were isolated using MACS® technology.