A Fed-Batch Chemically Defined hMSC-EV Bioprocess Medium Enabling 2-4x EV Yield Improvements In Bioreactor Culture

By Terri Willstaedt, Amy Walde, Elie Zakhem, Ph.D., and Jon A. Rowley, Ph.D.

Producing extracellular vesicles (EVs) from human mesenchymal stromal cells (hMSCs) often involves complex, multi-step processes that are inefficient and costly.​ A new chemically defined fed-batch bioprocess medium addresses these challenges by combining cell expansion and EV collection into a single streamlined workflow.​ This innovative approach eliminates the need for medium exchange and wash steps, reducing raw material usage and costs while expanding the EV collection window.​

The result is a 2-4x increase in EV yields compared to traditional methods, with preserved EV quality attributes such as size, tetraspanin expression, and RNA content.​ Tested across multiple donors and tissue types, this system demonstrates consistent performance and scalability to larger bioreactors. Download the poster now to explore how this breakthrough can optimize your hMSC-EV production process.