A Fed-Batch Chemically Defined hMSC-EV Bioprocess Medium Enabling 2-4x EV Yield Improvements In Bioreactor Culture

By Terri Willstaedt, Amy Walde, Elie Zakhem, Ph.D., and Jon A. Rowley, Ph.D.

Most hMSC-EV bioreactor processes still depend on an expansion phase with undefined components, followed by a medium exchange and washes before collection in a defined medium. This poster from RoosterBio scientists Terri Willstaedt, Amy Walde, Madeline Cramer, Stephen Lenzini, Elie Zakhem, and Jon A. Rowley describes a chemically defined fed-batch medium that carries cell expansion and EV collection through in a single step.

The data compare it with a traditional expand, wash, collect process across bone marrow and umbilical cord donors in spinner flasks, then extend to a 3 L stirred tank bioreactor. Yield gains are reported alongside size, tetraspanin expression, CD73 activity, and macrophage polarization data. Examine the figures to see how the results held across donors and scale.