A Complete Macrophage Suppression Assay Workflow

By Thibaut Janss, Juliette Lamy, Sofie Pattijn, Jana Schockaert

Scientists from ImmunXperts have used peripheral blood mononuclear cells (PBMCs) from three healthy donors in combination with Miltenyi Biotec products to perform an experiment validating a macrophage suppression assay workflow. Macrophages are a major component of the tumor microenvironment and orchestrate various aspects of immunity. Depending on their activation status, macrophages can exert dual influences on tumorigenesis.

The study demonstrates a consistent in vitro macrophage suppression assay using human primary immune cells which can be performed to analyze drug efficacy on macrophage polarization and suppression capabilities.