ABOUT BERKELEY LIGHTS
Berkeley Lights is a leading Digital Cell Biology company focused on enabling and accelerating the rapid development and commercialization of biotherapeutics and other cell-based products for our customers. The Berkeley Lights Platform captures deep phenotypic, functional and genotypic information for thousands of single cells in parallel and can also deliver the live biology customers desire in the form of the best cells.
APPLICATION NOTES
-
![scientist-vial]( "Screen Candidates And Select Better Leads For Developing Therapeutic Antibodies")
We demonstrate a workflow that can be used to access broad plasma cell diversity and rapidly down-select lead candidates with functional profiling.
-
![Berkeley AppNote_IND_RA]( "Achieve FDA-Accepted Monoclonality Assurance In Cell Line Development")
The Opto Cell Line development workflow provides >99% probability of clonality from a single automated round of cloning, while in-process imaging provides direct evidence of clonal derivation.
-
![App_Note_Aggregation Assay_RA]( "Improve Product Quality As Early As Clone Selection")
Product aggregation is a major stability, efficacy, and safety concern in the manufacturing of antibody therapeutics. This assay enables detection of product aggregates within days of single cell cloning.
-
![Berkeley Fig 4]( "Directly Link Phenotype To Gene Expression To Design Better Therapies")
Understanding the genetic mechanisms of T-cell polyfunctionality could be critical for developing effective therapeutics. This workflow makes it possible to identify these mechanisms.
-
![antigen presenting cell segregates the cytokines iStock-1146505074]( "Multidimensional Analysis Of T Cell Cytotoxicity And Serial Killing")
Interrogation of antigen-specific serial killing activity of single CAR T cells correlated to cytokine secretion and proliferation while preserving individual T cells for downstream analyses or expansion.
-
![NK Cell destroying a cancer cell iStock-1250270666]( "Simplify Single Cell Natural Killer Cell Cytotoxicity & ADCC Mediation")
This application note demonstrates how NK cell function can be assessed at the single-cell level to characterize both cytotoxicity and ADCC mediation in the presence of target cells.
-
![DNA Molecule Structure iStock-1186615180]( "Directly Link T Cell Phenotype And Function To Genotype")
Current techniques used to assess T cell function only allow us to draw correlative conclusions. This application note demonstrates how the Berkeley Lights platform can directly link phenotype to genotype to identify differences in CAR T cell killing kinetics as well as to recover TCR sequences that are associated with desired killing behavior.
-
![T cell cancer research iStock-981878366]( "Identify And Select Optimal CAR T Phenotypes")
This application note demonstrates how the Berkeley Lights platform can be used to identify single T cells that maintain proliferative ability while secreting specific cytokine combinations and exhibiting desired tumor killing activity.
VIDEOS
-
![Berkeley]( "Get to Market Faster With The Opto™ CLD Workflow")
Bring better and more molecules to market and access millions in enabled revenue.
-
![T cell attacking cancer cell iStock-1267123288]( "Multi-Omic Profiling Of T Cells Using The OptoSeq Single Cell 3' mRNA Kit")
Dr. Jason McEwen, the Director Biology at Berkeley Lights, reviews how to use a multi-omic workflow to find and fully profile polyfunctional T cells for more effective therapies.
-
![proteins with lymphocytes , t cells or cancer cells iStock-1139783322]( "How Pediatric Immune Systems Empower Cancer Immunotherapy")
Dr. David M Barrett, one of the developers of CAR T therapy in children with leukemia, shares new insight into Naïve T cells and how chemotherapy impacts their function.
-
![T cells attacking cancer cells iStock-1065829304]( "Expand And Rapidly Validate Antigen-Specific Function Of T Cells With Fewer Cells")
Immunogenicity validation is a critical bottleneck in the tumor antigen discovery process in part because the vast majority of candidate neoantigens identified in a tumor are not recognized by T cells. This product tour shows how the Opto™ Antigen Presenting Bead Kit and Opto™ Cell Therapy Development workflow address this bottleneck.
CONTACT INFORMATION
Berkeley Lights
5858 Horton St, Suite 320
Emeryville, CA 94608
UNITED STATES
Phone: 510-585-2855
PRODUCTS AND SERVICES
- Opto® Memory B Discovery Rabbit Workflow
- Opto™ Plasma B Discovery 4.0 Workflow
- Recover Clones With 99% Monoclonality Assurance And Reduce Timelines
- Develop A Deeper Understanding Of T Cell Biology
- T Cell Biology Reimagined
- Maximize Functional Characterization Of Your Precious T Cell Samples With The Opto™ Cell Therapy Development Workflow
- Get To Market Faster With The Opto™ CLD Workflow On The Beacon® System
- Beacon® Optofluidic System Brochure
- Increase CDMO Revenue With The Opto™ CLD Workflow On The Beacon® System
- Antigen Presentation Bead Kit
- Opto™ Cell Therapy Development Workflow: Single Cell Cytokine Assay
- Opto™ Cell Therapy Development Workflow: Cytotoxicity Assay
- Lightning™ Optofluidic System Brochure
- Opto™ Cell Therapy Development Workflow: TCRseq Well Plate Kit
BROCHURES
-
![T cell cancer research iStock-981878366]( "Opto® Memory B Discovery Rabbit Workflow")
Learn about an Optofluidic System that enables rapid selection of lead molecules by function-forward screening of B cells from immunized animals in under 1 week.
-
![GettyImages-1327623919-lab]( "Opto™ Plasma B Discovery 4.0 Workflow")
The Opto Plasma B Discovery 4.0 workflow on the Beacon® optofluidic system enables functional profiling of the B cell repertoire to rapidly advance lead molecules against difficult targets.
-
![iStock-1180568371-antibody-3D]( "Recover Clones With 99% Monoclonality Assurance And Reduce Timelines")
Explore how this optofluidic system offers an integrated end-to-end automated workflow for rapid cloning and selection in mammalian cell line development.
-
![proteins with lymphocytes , t cells or cancer cells GettyImages-1138937146]( "T Cell Biology Reimagined")
Our T cell workflow allows you to investigate complex cell-cell interactions. Our portfolio of capabilities includes assays to study polyfunctionality, cytotoxicity and the ability to directly link phenotype to gene expression.
-
![CTD_Workflow newest]( "Maximize Functional Characterization Of Your Precious T Cell Samples With The Opto™ Cell Therapy Development Workflow")
The Opto™ Cell Therapy Development worfklow accelerates the creation of better therapeutics by enabling multiple functional assays to be performed simultaneously on 1000s of individual T cells in a matter of days. Deeply characterize, profile, and map your T cell populations at the single-cell level to directly link behavior to gene expression.
-
![Berkeley]( "Get To Market Faster With The Opto™ CLD Workflow On The Beacon® System")
Find out how the Opto™ Cell Line Development workflow sets a new standard for quality, capacity, and speed in cell line development to let BioPharma get more molecules to the clinic faster.
-
![Beacon]( "Beacon® Optofluidic System Brochure")
Identifying T cell functional signatures is a long, laborious, and complex process that requires you to draw conclusions from surrogate measurements collected on multiple platforms. The Lightning system changes this by allowing you to directly visualize the phenotype and function of 1000s of individual cells in just days and recover cells of interest alive.
-
![Berkeley]( "Increase CDMO Revenue With The Opto™ CLD Workflow On The Beacon® System")
Find out how the Opto™ Cell Line Development workflow sets a new standard for quality, capacity, and speed in cell line development and allows CDMOs to scale operations and drive new business.
-
![APB]( "Antigen Presentation Bead Kit")
Antigen-specific T cells can be selectively expanded using the Opto™ Antigen Presenting Bead kit upstream of the Opto™ Cell Therapy Development workflow, enabling you to directly link peptide binding and recognition to antigen specific effector function.
-
![T-cell]( "Opto™ Cell Therapy Development Workflow: Single Cell Cytokine Assay")
Cytokine assays in the Opto™ Cell Therapy Development workflow allow simultaneous functional interrogation of thousands of individual T cells as they interact with antigen-presenting cells, granting insight into the heterogenous mechanisms that regulate cytokine secretion. The automated assays also allow live recovery of individual clones for downstream analysis. Cytokine assays and cell surface markers can be customized.
-
![Cancer Tumor]( "Opto™ Cell Therapy Development Workflow: Cytotoxicity Assay")
The Opto™ Cell Therapy Development workflow Cytotoxicity Assay enables kinetic analysis of killing activity from single T cells, followed by live cell recovery for genomic analysis. This assay avoids common problems associated with traditional killing assays, which measure average target cell lysis at fixed time points, obscuring kinetic details and ignoring the heterogeneity present in T cell subsets.
-
![Berkeley Lightning with People_241-edit2]( "Lightning™ Optofluidic System Brochure")
Identifying T cell functional signatures is a long, laborious, and complex process that requires you to draw conclusions from surrogate measurements collected on multiple platforms. The Lightning system changes this by allowing you to directly visualize the phenotype and function of 1000s of individual cells in just days and recover cells of interest alive.
-
![iStock-1216303741-scientist-lab]( "Opto™ Cell Therapy Development Workflow: TCRseq Well Plate Kit")
Used downstream of functional T cell assays in the Opto™ Cell Therapy Development workflows, the TCRseq kit enables recovery of functionally validated T cell receptors (TCRs) from fewer than 10,000 input cells. Instead of re-expressing hundreds of TCRs in the hopes of identifying the rare sequence that imparts ideal specificity, the kit allows users to only sequence the T cells that matter.
