IMPORTANCE OF DATA REPRODUCIBILITY FOR DEVELOPMENT OF SAFE GENE THERAPIES

Viral-based vectors have now become an integral part of clinically approved gene therapies. Ongoing optimization of AAV vector efficiency and specificity, as well as the discovery of new adenovirus strains in nature, offer opportunities for new AAVs to be explored as potential therapeutic constructs. New vector strains and designs have the potential to introduce genetic elements that elicit an immune response, so they must be investigated for both efficacy and safety. When the therapeutic product, viral capsid, or other vector elements may be culprits in gene therapy-induced toxicity, exploring activation or silencing of gene expression may help to guide vector design and construction to balance therapeutic efficacy against potential toxicity.

Follow along with an introduction to the importance of reproducible quantitation of gene expression to investigate potential sources of vector-induced immunotoxicity in gene therapy, along with a demonstration of well-to-well and instrument-to-instrument data concordance across multiple biosystems.

FEATURED INSIGHTS

Real-Time PCR And Gene Expression Specificity

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Efficient Gene Expression Profiling For Biomarker Identification

Solutions for automating qPCR and digital PCR (dPCR) gene expression analysis workflows can help translational research laboratories derive valuable gene expression profiles.
How To Evaluate And Compare Real-Time PCR Reactions

Learn about the key factors that influence qPCR reaction performance and provides guidelines for evaluating and comparing reactions run under varying conditions to ensure reliable data interpretation.
Guidelines For Comparing SYBR Green Master Mixes

Explore a protocol for comparing the performance of Applied Biosystems PowerTrack SYBR Green Master Mix with other master mixes in PCR applications.
Maximize Your Gene Therapy Process With Genetic Analysis

Propel your gene therapy research to the forefront of discovery by incorporating genetic analysis into your investigations at any phase of the development process.
Importance Of Data Reproducibility For Developing Safe Gene Therapies

What are viral-based vectors and why are reproducible quantitation of gene expression important in gene therapy? Explore these questions as well as the interplay among gene therapy vector components.
Considerations For Cell Therapy Development And Manufacturing

Review the latest methodologies, common practices, resources, applications and more to support every step of your cell therapy manufacturing workflow.
Leverage qPCR And dPCR To Streamline Biologics Development

PCR methods that deliver high-quality results throughout biologics development and biomanufacturing can help biologics manufacturers bridge the gap from basic research to clinical trials and manufacturing.
QualTrak Real-Time PCR And Digital PCR Ecosystem For Streamlined Biologics Development

A vendor who provides high-quality qPCR and dPCR products—assays, reagents, and instruments for analysis—is vital for the success of biologics development.
Uniformity Of Gene Expression Measurements On The QuantStudio 7 Pro Real-Time PCR System

We demonstrate a system that combines all of the qPCR features scientists want in a single high-performance instrument so that you can optimize your research productivity.