What Is Transfection?

Transfection, the process of introducing nucleic acids into cells, is a fundamental tool in modern biological research. It enables scientists to study gene function, develop new therapies, and engineer tissues. While viral transduction is a popular method, chemical transfection using reagents like calcium phosphate, polycations, and liposomes remains a cornerstone.

The success of transfection depends on various factors, including cell type, nucleic acid quality, and reagent choice. To optimize transfection efficiency, researchers often experiment with different parameters like DNA:reagent ratio, complex formation time, and post-transfection incubation.

By understanding the principles of transfection and following best practices, scientists can effectively manipulate gene expression and unlock new insights into cellular biology. Learn more about transfection by reading the full article, available below.