Visualizing The Metabolic Profile Of Immune Cells With Metabolic Inhibitors Using LiCellMo™, A Live Cell Metabolic Analyzer

Immune cell function and therapeutic potency are intimately linked to metabolic state, yet traditional discrete sampling fails to capture dynamic cellular responses to metabolic inhibitors. Continuous in-line measurement of glucose and lactate levels reveals how distinct immune cell lines alter their energy pathways over time. Evaluating acute leukemia models demonstrates that targeted inhibition of glycolysis rapidly suppresses metabolic rates in glycolysis-dependent lines, whereas mitochondrial inhibition triggers compensatory metabolic shifts. Because sensor measurements align within 9% of traditional colorimetric assays, this approach offers precise, non-invasive profiling of cellular responses throughout activation, proliferation, and exhaustion phases. Access the complete application note to explore detailed metabolic profiles and inhibitor response curves.