Vericheck ddPCR Empty-Full Capsid Kits

Adeno-associated virus (AAV) has become the preferred vector for gene therapy and assessing the ratio of empty to full capsids is a critical and required step in AAV production. Previously, a significant amount of precious sample was required. Now, with the absolute quantification of Droplet Digital™ PCR, the Vericheck ddPCR Empty-Full Capsid Kit requires only small volume of crude lysate or purified samples, which is ideal for any step in the AAV vector development or manufacturing process.

The Vericheck ddPCR Empty-Full Capsid Kit enables a quick and precise measurement of the empty-full capsid ratio that is needed to produce AAV vectors. The empty-full capsid ratio is a Critical Quality Attribute (CQA) in AAV vector production since high amounts of empty capsids may cause unwanted immunotoxicity. This kit simultaneously provides consistent and reliable capsid content measurements across the AAV production process with high precision, multi-sample type compatibility, low-sample input, fast turnaround time, and easy data analysis.

Features & Benefits

• Sample type compatibility — crude lysate and purified
• Low sample input — only 10⁶ virus particles required to get results
• Fast turnaround time — up to 14 samples per plate in less than 1 day
• High precision — coefficient of variation (CV) less than 5%
• Simplified data analysis – streamlined process providing simultaneous results for genome titer, capsid titer, and empty-full ratio

Applications & Uses

• Simply and precisely measure empty-full ratio with one assay
• Optimized to provide precision throughout the AAV characterization process