Use Of 60 Micron Plastic (PE) Retention Filter For Perfusion MX With PBS-3 VW Bioreactor

Retention filters are crucial for perfusion medium exchange (MX) in Pluripotent Stem Cell (PSC) cultures, using Vertical-Wheel Bioreactors. This application note offers guidance on assembling the PBS-3 perfusion system with a 60 Micron Plastic (PE) Retention Filter.

This document outlines the required materials and provides detailed, step-by-step instructions for the construction of the spent medium line, fresh medium line, and medium reservoir cap configuration. It also covers the complete perfusion system setup, including the integration of the retention filter and dip tube.

Additionally, instructions are provided on initiating perfusion MX, replacing media, and priming the system. It also offers guidance on pump setup and calibration, and recommends ideal flow rates based on working volume and MX period.

Access the full application note to optimize your PSC culture process in Vertical-Wheel Bioreactors using the PBS-3 perfusion system.