The New Gold Standard For Gene Therapy Protein Expression Potency Measurement

Simple Western is helping transformative gene therapies reach patients faster. As a fit-for-purpose tool for quantitative gene therapy potency assays to quantify vector-mediated protein expression, Simple Western works with a variety of complex sample matrices with minimal matrix effects.

Compared to traditional methods like ELISA and Western blot, Simple Western requires much smaller protein loads, preserving precious tissue samples. Simple Western was tested to demonstrate a it's relative potency assay with parallel line analysis (PLA) for AAV-mediated gene delivery in cultured human cells. By measuring GFP normalized to total protein directly in transduced cells, this application shows that AAV2 is significantly more potent than AAV9 with highly reproducible quantitation that is 10X more sensitive than flow cytometry, with improved specificity and less matrix effect than ELISA.