Tangential Flow Filtration (UF/DF) Of Plasmid DNA

Due to their structure, plasmids can sometimes pass through pores that are smaller than their apparent molecular weight. This sieving can be more predominant with flux increase and its coefficient also increases at higher ionic strength due to reduction in the effective plasmid size observed in these conditions.

Additionally, the DNA can be shear-sensitive and tends to increase with plasmid size, and the result can be degradation and reduction of the overall yield.

Read this application note to learn more about how optimization of these hydraulic parameters can help you achieve the desired plasmid purity, formulation, and concentration specifications.