Synthetic sgRNAs Enable Researchers To Study Viral Infection In Resting Human CD4+ T Cells

Are you facing challenges with maintaining the resting states in CD4+ T cells subsequent to gene knockout? Failing to do so can limit the analysis of pathways and factors that trigger CD4+ T cell activation. Synthego provides an optimized approach to editing primary human resting CD4+ T cells that can help resolve this issue. The combination of Synthego's highly efficient synthetic sgRNAs, Lonza’s 4D-Nucleofector^®, and optimal culturing conditions can ensure high cell survival following Nucleofection^®.

In this study, researchers targeted multiple genes related to viral infectivity, knocked in large reporter gene segments, and performed downstream functional assays to characterize their various gene edits. Review how Synthego’s synthetic single guide RNAs play a role in enabling high-efficiency knockouts in resting CD4+ T Cells.

Download this application note below and check out the rest of Synthego's Discovery Resource Collection:

• Synthetic Research Grade sgRNA Manufacturing For Your CRISPR Experiments
• High Efficiency T-Cell Editing- Utilizing CRISPRevolution™ Synthetic sgRNA
• CRISPR 101 - Your Guide To Understanding CRISPR