|
|
|
|
Accurate lentiviral characterization is essential to help ensure safety, efficacy and regulatory compliance in cell and gene therapy. In this session, QIAGEN and Niba Labs experts present a streamlined dPCR workflow for quantifying viral genome titers, vector copy number (VCN), and replication-competent lentivirus (RCL), supporting standardized, high-precision quality control.
|
|
|
|
|
Reliable lentiviral titer determination is a critical quality attribute throughout development and manufacturing. This resource introduces a streamlined one-step RT-dPCR workflow that replaces traditional RNA extraction with direct lysis, reducing hands-on time while enabling precise and reproducible quantification for process optimization.
|
|
|
|
|
Accurate vector copy number analysis is essential for safe and consistent CAR-T development. This collaborative study with Amsterdam UMC demonstrates a flexible multiplex dPCR approach combining custom viral and transgene assays with established reference targets. The workflow enables simultaneous quantification of multiple targets in a single reaction, supporting robust and reproducible VCN analysis for cell therapy applications.
|
|
|
|
|
VCN is a critical quality attribute for gene-modified cell therapies, impacting safety, efficacy and regulatory compliance. This application note highlights a direct cell lysis workflow that simplifies sample preparation by replacing conventional DNA extraction. Combined with dPCR, the method enables rapid, precise and reproducible VCN quantification across a wide range of cell inputs, supporting scalable cell therapy workflows.
|
|
|
|
|
|
|
Learn how dPCR enables accurate measurement of critical quality attributes without standard curves, improving sensitivity, reproducibility and confidence in results. This talk highlights how QIAcuity supports scalable workflows and regulatory-compliant environments from development to GMP manufacturing.
|
|
|
|
|
|
|
Discover how dPCR enables accurate and reproducible quantification of critical quality attributes across cell and gene therapy workflows. From vector copy number and viral titer to impurity and safety testing applications, dPCR supports robust quality control and confident decision-making from development through manufacturing.
|
|
|
