Optimizing ex vivo cell expansion processes in preparation for clinical use is a critical step in cell therapy manufacturing. Given the curative and lifesaving impacts these therapies can have on patients, performance and quality are essential outputs of any expansion process. Here, we discuss the performance of Stemline® XF MSC Medium, which promotes expansion of human mesenchymal stromal/stem cells (hMSCs) to high densities while maintaining cell identity and quality. This product was designed for efficient expansion in planar and microcarrierbased culture platforms, easing the transition between research, clinical, and manufacturing scale culture. We describe the ex vivo expansion of bone marrow-derived hMSCs cultured in Stemline® XF MSC Medium in a 3 L stirred tank bioreactor-microcarrier platform.