Stable Cell Lines For Lentivirus And Gammaretrovirus Production

The aim of upstream processing (USP) in viral vector (VV) development and manufacturing is to generate a suitable quantity of vectors while ensuring that purification in downstream processing (DSP) can be achieved efficiently. One key decision that must be made prior to USP during lentiviral and gammaretroviral vectors is whether to rely on transient transfection or stable producer cell lines. 

Read to learn the advantages and disadvantages of transient transfection and explore the reasons why many VV developers are keen to move towards the use of stable producer cell lines for lentiviral vector (LV) development.