Single Channel Pipette Calibration And Operator Competency Assessment Using A Dual-Dye Ratiometric Photometry System

By Rebecca L. Kitchener, PhD and Nathaniel G. Hentz, PhD., Artel, Inc.

Handheld pipettes are the workhorse of most life sciences laboratories, and they are among the most ubiquitous laboratory instrumentation. They are the single most frequently utilized of all calibrated laboratory instruments, yet they are often overlooked as potential sources of inaccuracy and imprecision in method development and validation. Proper pipette performance is essential to the generation of data that are both reliable and reproducible – two hallmarks of a laboratory’s reputation and success. Discover a pipette calibration system that employs the principle of dual-dye ratiometric photometry for immediate volume verification.