Application Note

Single Cell Seeding Conditions For Improved Colony Outgrowth

Advanced Instruments VIPS_Lab_Photo

Determining the best conditions possible for successful single cell outgrowth can be a jigsaw puzzle of factors that include the host cell line, the transfection conditions, method, vector, selection and recovery period before seeding and even the numerous growth condition factors following seeding such as plate type, plate coatings, media types, media volumes

In this paper we present data that shows the importance of considering the numerous factors that can affect single cell outgrowth when optimising growth conditions. The seeding method and instrumentation can dramatically improve the seeding efficiency from the start, but if the conditions and environment surrounding the single cell are not optimal then subsequent outgrowth and cloning efficiency will always be a challenge to improve. The combination of the VIPS and InstiGRO CHO products have dramatically and consistently increased the percentage of clonal outgrowth achieved in these experiments.

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