Simplifying Adenoviral Purification For Scalable Manufacturing

Adenoviral vectors are mature and well characterized, yet purification still sets the ceiling on yield, cost, and efficiency. Conventional chromatography workflows meet regulatory expectations, but cumulative losses across steps, heavy buffer demand, and long processing times persist.

This paper makes the case for reduction over expansion, describing a membrane-integrated hybrid train that works for both conventional and helper-dependent systems. It sets reported recovery, impurity clearance, buffer use, and cycle time against conventional workflows in a side-by-side comparison. It also looks at how impurity profiles shift between bench and 500 L scale, including a representative case of breakthrough and fouling and the process adjustments that resolved it.

Explore the data and see how process architecture shapes manufacturing economics.