Simplified Workflow Using Automated Instrument Setup And Compensation On The BD FACSLyric™ Flow Cytometer

By Casey J Fox, Mamatha Bharadwaj, Dina Huckaby, Maclean Nyachienga, Bao Nguyen, Rebecca Hershock-Quintana, Darci Gorgone Chavez, Nathan Whizin, and Alan Stall

Flow cytometry is a powerful technique for the single-cell analysis of patient samples. As the scope and complexity of clinical flow experiments continue to grow, the demand for instruments that offer both exceptional performance and ease of use increases. This application note highlights the usability and performance capabilities of the 10-color CE-IVD BD FACSLyric™ Flow Cytometry System. In combination with BD FACSuite™ Clinical Application, BD® Cytometer Setup and Tracking (CS&T) Beads, and BD® FC Beads, the system provides a streamlined workflow, from instrument initialization to data acquisition and analysis.

The reproducibility of median fluorescence intensity (MFI) over time, across multiple cytometers, and alongside resolution sensitivity was assessed using BD® CS&T Beads and stained cells. Additionally, fluorescence spillover reproducibility was evaluated using BD® FC Beads and stained cells. To further investigate the system's accuracy and reliability during automated cytometer setup, the performance of three to six 10-color BD FACSLyric™ Flow Cytometers over a two-month period was measured. Lastly, the overall performance of the system was tested using a multicolor assay to identify T, B, and NK cells in peripheral blood from both HIV patients and control subjects. Overall, the findings demonstrate that the BD FACSLyric™ System offers clinical users valuable tools for producing consistent and reproducible results across both time and instruments.