A Comprehensive Suite Of On-Target And Off-Target Assessment Assays
Our comprehensive off-target gene editing assessment platform integrates screening, nomination, and confirmation assays, providing unmatched precision in guide RNA selection and off-target risk assessment. By addressing safety and regulatory expectations early, our platform reduces development risks, streamlines therapeutic design, and accelerates your path to the clinic with confidence and efficiency.
Screening Assays: Preview of off-target risk for informed guide selection
Guide Profiler™
In silico preview of potential off-target effects associated with your gene editing tool. Enables you to start your gene editing risk assessment by rapidly screening sgRNAs to understanding on-target efficacy and off-target editing risks.
Guide Select™
Off-target assessment assay designed for screening multiple guide RNAs in parallel. Variant-aware searching within a limited search space enables high-powered, low-cost risk assessment for guide RNA selection.
Nomination Assays: Comprehensive off-target assessment
ONE-seq™
Gold-standard, population-scale, variant-aware, off-target detection. Candidate off-target enumeration, enrichment, and biochemical in vitro cutting combined with deep sequencing enables high sensitivity detection of low frequency off-target events.
DEUX-seq™
Orthogonal approach to supplement ONE-seq off-target nomination. Unbiased biochemical off-target editing assay provides a complementary approach to supplement your off-target risk assessment efforts.
GUIDE-seq™
Orthogonal approach to supplement ONE-seq off-target nomination. Unbiased cell-based off-target editing assay provides a complementary approach to supplement your off-target risk assessment efforts.
Confirmation Assays: Verifies cellular edits and structural rearrangements
Amplicon-seq™
High sensitivity confirmation of off-target effects in your cell type of interest post-editing. Informed by nomination assays, NGS pinpoints off-target edits with resolution down to exact genomic coordinates.
SAFER Detection™
High sensitivity confirmation of structural rearrangements in your cell type of interest post-editing. NGS interrogation allows for resolution down to exact genomic coordinates.