Scaling Up Your E. Coli pDNA Process From Parameter Screening To Pilot Scale With Advanced Single-Use Bioreactors

Plasmid DNA (pDNA) production plays a pivotal role in modern biotechnology, underpinning a wide range of applications including gene therapy, vaccine development, and genetic engineering. Achieving efficient and scalable pDNA production requires a methodical approach to process development, with a focus on optimizing key parameters and implementing effective scale-up strategies to ensure high yields and consistent productivity. This webinar outlines the development and scale-up of a pDNA production process using Escherichia coli (E. coli) OneShot Top10 as the host strain. The process began with a structured design of experiments (DOE), specifically a 4-factor, 2-level screening study, to identify the most influential parameters affecting culture performance and pDNA yield. Based on the insights gained from the DOE, the optimal conditions were validated in a 1-liter fermentation setup to confirm their effectiveness at a small scale.

This comprehensive and systematic approach to pDNA production emphasizes the importance of experimental design, parameter optimization, and thoughtful scale-up in achieving reproducible and efficient outcomes in biomanufacturing.