Resistance And Chemical Inactivation Efficacy Of AAV And Other Parvoviruses

Adeno-associated viral (AAV) vectors are vital to gene therapies, yet they fall outside the scope of most established disinfectant efficacy testing guidelines. This uncertainty means that the effectiveness of commercially available disinfectants against AAV has not been systematically validated. Traditionally, Porcine Parvovirus (PPV) and Minute Virus of Mice (MVM) have served as surrogates for AAV in validation studies due to their structural similarities. However, the predictive value of these surrogates remains uncertain.

A study evaluating the chemical inactivation efficacy of five common disinfectants against AAV2, PPV, and MVM found that MVM's resistance profile closely mirrored that of AAV2. In contrast, PPV showed a consistently higher susceptibility to all tested disinfectants, suggesting that its use in isolation may overestimate disinfectant efficacy. With the exception of Hydrogen Peroxide (2%), all disinfectants achieved ≥log10 reduction against the parvoviruses within the defined contact times. This indicates that MVM may be a more appropriate surrogate for AAV in high-level disinfectant validation, thereby improving the relevance and reliability of contamination control protocols in manufacturing.

Explore a detailed analysis of the inactivation kinetics across all five disinfectants and exposure times.