Rapid Production Of Assay Ready Cells For GPCR Drug Discovery

G-protein coupled receptors (GPCRs) regulate various physiological processes and are targets for many existing drugs. However, developing stable cell lines expressing GPCRs for drug discovery can be challenging due to expression drift. An alternative approach is to use transiently transfected Assay Ready Cells, which can be prepared rapidly using electroporation in physiologically relevant host cell types. This method allows for fine-tuning the sensitivity of GPCR assays by varying plasmid DNA concentrations.

This enables researchers to achieve superior performance with reduced day-to-day assay variability across different scales, accelerating drug discovery efforts. Learn more about how to develop GPCR assays with proven performance by accessing the full application note.