Prepare For Successful Biomarker Profiling From Cell-Free DNA

Cell-free DNA (cfDNA), which is released into the bloodstream or other body fluids by both healthy and diseased individuals, has emerged as a critical analyte in the field of liquid biopsy. These circulating DNA fragments offer a non-invasive window into the genetic landscape of an individual, which reveals a wide array of genomic alterations. These include single-nucleotide variants, insertions and deletions, and larger chromosomal abnormalities such as copy number changes and translocations. cfDNA profiling is also increasingly applied in oncology, prenatal testing, and transplant monitoring, underscoring its broad clinical utility. Beyond these, additional layers of information—such as structural variants and epigenetic modifications like DNA methylation—can further enrich cfDNA analysis, offering a more comprehensive view of disease states and biological processes.

To ensure the reliability and reproducibility of cfDNA-based analyses, it is essential to standardize the methods used for sample collection and preparation. In response to this need, our expert scientists have compiled a set of technical guidelines that reflect best practices and practical recommendations for preanalytical handling of cfDNA. These recommendations address crucial steps such as sample stabilization, centrifugation timing, and DNA extraction to help minimize variability and DNA degradation. These guidelines are designed to support researchers and clinicians in achieving high-quality, consistent results across studies and clinical applications.

Access these expert-driven recommendations to strengthen your cfDNA workflows across research and clinical settings. Learn how you can contribute to more accurate diagnostics and better patient outcomes by adopting standardized practices.