Preparative Anion Exchange Chromatography For The Purification Of Phosphorothioate DNA Oligonucleotides

By Anton Posch, Franziska Kollmann, Joseph Poblete, Sharon Bola, Khaled Mriziq, and Elizabeth Dreskin

Incomplete or unusual sequences of oligonucleotide (ON) during solid-phase synthesis are key impurities that need to be removed before these molecules can be used as therapeutics. The production of pure therapeutic DNA ONs requires efficient, scalable, and cost-effective chromatographic purification methods applied after chemical synthesis.

This study explores how preparative AEX chromatography with Nuvia HP-Q Resin compares to the typical chromatography utilized in removing length-based impurities. Key process parameters such as flow rate, gradient slope, buffer pH, and dynamic binding capacity (DBC) were factors in the testing. Discover if the AEX Nuvia HP-Q Resin is well-suited for the purification of standard ONs with phosphodiester backbones.